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KMID : 0861020080230040113
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Korea Journal of Herbology
2008 Volume.23 No. 4 p.113 ~ p.120
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Anti-atherosclerosis Effect of Imyosan Extract in Human Aortic Smooth Muscle Cells
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Yun Hyun-Jeong
Heo Sook-Kyoung
Yi Hyo-Seung
Kim Tae-Hoon
Kim Dong-Wan
Kim Sun-Mo
Park Sun-Dong
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Abstract
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Objectives: This study was evaluated to elucidate the inhibitory potential of Imyosan(IMS) and its components, Phellodendri Cortex(PC: Phellodendron amurense Rupr., Hwangbaek in Korean) and Atractylodis Rhizoma(AR: Atratylodes lancea D.C., Changchool in Korean), on human aortic smooth muscle cells(HASMC) migration and production of matrix metalloproteinase (MMP)-2 and MMP-9 by TNF- treatment.
Methods: Cytotoxic activity of IMS and its components on HASMC was using 5-(3-caroboxy meth-oxyphenyl)-2H-tetra-zolium inner salt(MTS) assay. Effect of IMS, PC and AR on TNF--induced HASMC migration underside of matrigel filter was stained with hematoxylin-eosin. And total number of cells that migrated to the underside of the filter was counted. MMP-2 and MMP-9 activity was evaluated by gelatin zymography assay.
Results: The matrigel migration assay showed that IMS effectively inhibited the TNF--induced migration of HASMC. Moreover, IMS significantly inhibited MMP-9 activity. Our present study demonstrates that IMS and its components inhibit TNF--induced HASMC migration and MMP-9 activity. The inhibitory effect of IMS extract is more potent than that of its component herb extracts.
Conclusions: These results provide evidence that IMS has multiple effects in the inhibition of HASMC migration and may offer a therapeutic approach to block HASMC migration.
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KEYWORD
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Imyosan(IMS), Phellodendri Cortex(PC), Atractylodis Rhizoma(AR), migration, matrix metalloproteinase(MMP)
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